›› 2012, Vol. 24 ›› Issue (1): 56-59.doi: 10.3969/j.issn.1004-616x.2012.01.013

• 肿瘤防治 • 上一篇    下一篇

三氯乙烯药疹样皮炎病人外周血c-fos、c-myc、K-ras和p53 mRNA表达水平的研究

徐新云，刘月峰，易 娟，周 丽，黄新凤，毛吉炎，毛侃琅   

  1. 1. 深圳市疾病预防控制中心，深圳 518055；2. 深圳市职业病防治院，深圳 518001
  • 收稿日期:2011-05-19 修回日期:2011-09-16 出版日期:2012-01-30 发布日期:2012-01-30
  • 通讯作者: 徐新云

mRNA expression of oncogenes in patients with allergic dermatitis induced by trichloroethylene

XU Xin-yun，LIU Yue-feng，YI Juan，ZHOU Li，HUANG Xin-feng，MAO Ji-yan，MAO Kan-lang   

  1. 1. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055; 2. Shenzhen Hospital for Occupational Disease Prevention and Treatment, Shenzhen 518001, Guangdong, China
  • Received:2011-05-19 Revised:2011-09-16 Online:2012-01-30 Published:2012-01-30
  • Contact: XU Xin-yun

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摘要： 目的： 探讨三氯乙烯 (trichloroethylene，TCE)药疹样皮炎病人外周血癌基因c-fos、c-myc、K-ras、p53 mRNA表达水平的变化情况。 方法：分别抽取4例健康人 (对照组)及4例三氯乙烯致变态反应病人 (病例组)抗凝外周全血，采用实时荧光定量PCR (real-time quantitative PCR)技术检测外周血中c-fos、c-myc、K-ras和p53 mRNA的表达水平。 结果：与健康对照者比较，三氯乙烯药疹样皮炎病人外周血c-fos mRNA表达水平升高352%，c-myc mRNA升高41%，K-ras mRNA升高136%，p53 mRNA升高64%，两组间上述基因mRNA表达水平的差异均有统计学意义 (P<0.01 或P<0.05)。结论：三氯乙烯药疹样皮炎病人外周血癌基因表达水平增加，提示三氯乙烯可能有一定的致癌风险。

关键词: 三氯乙烯, 外周血, 原癌基因, 荧光定量PCR, 致癌性

Abstract: OBJECTIVE: To study mRNA expression of oncogenes (c-fos，c-myc，K-ras，p53) in peripheral blood of patients allergic to trichloroethylene (TCE). METHODS：Peripheral blood samples were collected from healthy workers (control group) and allergic patients (case group). Real-time quantitative PCR was applied to detect mRNA expression of oncogenes c-fos，c-myc，K-ras，p53. RESULTS：The level of c-fos mRNA expression increased by 352% in TCE patients when compared with control (P<0.01)，c-myc increased by 41%，K-ras by 136% and p53 increased by 64%. mRNA expression levels of these oncogenes showed significant differences between case and control groups (P<0.01 or P<0.05). CONCLUSION：Trichloroethylene could induce oncogene expression in patients with allergic dermatitis， indicating that TCE might be potentially carcinogenic.

Key words: trichloroethylene, peripheral blood, oncogenes, real-time PCR, carcinogenicity

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